Unofficial Depth Chart September 22
| Off | First | Second | Third | Fourth |
| WR | T. Hill | M. Hardman | M. Kemp | - |
| LT | E. Fisher | M. Remmers | - | - |
| LG | K. Osemele | N. Allegretti | - | - |
| C | A. Reiter | D. Kilgore | - | - |
| RG | A. Wylie | Y. Durant | - | - |
| RT | M. Schwartz | M. Remmers | - | - |
| TE | T. Kelce | N. Keizer | R. Seals-Jones | D. Yelder |
| WR | S. Watkins | D. Robinson | B. Pringle | - |
| QB | P. Mahomes | C. Henne | - | - |
| RB | C. Edwards-Helaire | Dar. Williams | D. Thompson | - |
| FB | A. Sherman | - | - | - |
| Def | First | Second | Third | Fourth |
| LDE | T. Kpassagnon | A. Okafor | M. Danna | - |
| LDT | C. Jones | T. Wharton | - | - |
| RDT | D. Nnadi | M. Pennel | - | - |
| RDE | F. Clark | T. Charlton | D. Harris | - |
| LB | A. Hitchens | B. Niemann | D. O'Daniel | - |
| LB | D. Wilson | W. Gay | - | - |
| LCB | C. Ward | T. Keyes | - | - |
| RCB | R. Fenton | L. Sneed | - | - |
| CB | A. Hamilton | R. Fenton | - | - |
| FS | D. Sorensen | J. Thornhill | A. Watts | - |
| SS | T. Mathieu | T. Thompson | - | - |
| ST | First | Second | Third | Fourth |
| P | T. Townsend | - | - | - |
| K | H. Butker | T. Townsend | - | - |
| H | T. Townsend | - | - | - |
| LS | J. Winchester | - | - | - |
| PR | M. Hardman | T. Hill | - | - |
| KR | M. Hardman | T. Hill | - | - |
Changes since last depth chart: Mike Pennel replaced Khalen Saunders behind Derrick Nnadi at right defensive tackle.
Data from Chiefs.com, but is considered unofficial.